LC & LC/MS Columns 

LC and LC/MS Column Selection Flow Chart

To use the column selection guide diagram below, simply follow the path for your analyte and mobile phase. At the far right, click on your final column selection to view detail information.

SAMPLE	Molecular Weight < 2000	Organic Solvent Soluble	Hexane Soluble		Normal-Phase Mode with Bare Silica
					Normal-Phase Mode with Bonded-Phase
			MeOH and MeOH:H20 or ACN and ACN:H20 Soluble		Reversed-Phase Mode with Bonded Phase Gel-Permeation (Small Molecule)
			THF Soluble
					Gel-Permeation (Small Molecule)
		Water Soluble	Non-Ionic		Reversed-Phase Mode with Bonded Phase
			Ionic		Reversed-Phase Mode with Bonded Phase Using Ionization Control
					Reversed-Phase Mode with Bonded Phase Using Ion-Pair Agent
					Reversed-Phase Mode with Bare Silica
					Ion-Exchange Mode
	Molecular Weight > 2000	Organic Solvent Soluble			Gel-Permeation Chromatography
		Water Soluble			Gel-Filtration Chromatography
					Ion-Exchange Mode with Wide-Pore Material
					Reversed-Phase Mode with Wide-Pore Material