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Agilent Bio-Monolith HPLC Columns 

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Achieve rapid, high resolution separations of large bio-molecules 
The Agilent Bio-Monolith HPLC columns provide high resolution and rapid separations of antibodies (IgG, IgM), plasmid DNA, viruses, phages and other macro bio-molecules. The product family offers strong cation exchange, strong and weak anion exchange and Protein A phases. Bio-Monolith HPLC Columns are compatible with HPLC and preparative LC systems, including Agilent 1100 and 1200 HPLC systems.

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Features
  • Polymer-based, monolith HPLC columns allow for flow rate independent separations. No diffusion, no pores and no void volume make transport between mobile and stationary phase very rapid.
  • Monolith disc is 5.2 mm x 4.95 mm (100 µL column volume) with continuous channels, eliminating diffusion mass transfer.
  • Extremely fast separations speed up method development time and decrease costs. Lock in method parameters take significantly less time and less buffer.
  • Bio-Monolith QA - Strong anion exchange bonded phase fully charged over a working pH range of 2-13, binding bio-molecules with negative charges. Applications include process monitoring and quality control of IgM and adenovirus purifications.
  • Bio-Monolith DEAE - Weak anion exchange bonded phase offering increased selectivity of bio-molecules with negative charge over a working pH range of 3-9. Applications include process monitoring and quality control of bacteriophage and plasmid DNA purifications.
  • Bio-Monolith SO3 - Strong cation exchange bonded phase fully charged over a working pH range of 2-13, binding bio-molecules with positive charges. Applications include fast and high resolution analytical separations of large bio-molecules such as proteins and antibodies.
  • Bio-Monolith Protein A – The Protein A affinity column is designed for the analytical separation of all IgG (human and mouse), except for IgG class3. Enables rapid separation and quantitation of IgG.

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