Agilent | QuEChERS Dispersive SPE Kits

Step Two: Dispersive SPE Clean-Up

Select the Dispersive SPE kit suited to the type of food being analyzed and the method you are following. In this step, an aliquot of the sample extract from Step One is added to 2 mL or 15 mL centrifuge tube containing a small amount of SPE sorbent and MgSO₄. The sorbent will pull out intefering matrix materials from the sample, and the MgSO₄ helps remove excess water and improve analyte partitioning

Dispersive SPE Kits
Kit	Quantity & Size/Pack	AOAC 2007.01 Method	European Method EN 15662
Kit	Quantity & Size/Pack	Contents & Part No. CH - with Ceramic Homogenizers	Contents & Part No. CH - with Ceramic Homogenizers
General Fruits and Vegetables: Removes polar organic acids, some sugars and lipids	100 – 2 mL tubes	50 mg PSA 150 mg MgSO₄Part No. 5982-5022, 5982-5022CH	25 mg PSA 150 mg MgSO₄Part No. 5982-5021, 5982-5021CH
	50 – 15 mL tubes	400 mg PSA 1200 mg MgSO₄Part No. 5982-5058, 5982-5058CH	150 mg PSA 900 mg MgSO₄ Part No. 5982-5056,5982-5056CH
Fruits and Vegetables with Fats and Waxes: Removes polar organic acids, some sugars, more lipids and sterols	100 – 2 mL tubes	50 mg PSA 50 mg C18EC 150 mg MgSO₄Part No. 5982-5122, 5982-5122CH	25 mg PSA 25 mg C18EC 150 mg MgSO₄Part No. 5982-5121, 5982-5121CH
	50 – 15 mL tubes	400 mg PSA 400 mg C18EC 1200 mg MgSO₄Part No. 5982-5158, 5982-5158CH	150 mg PSA 150 mg C18EC 900 mg MgSO₄ Part No. 5982-5156, 5982-5156CH
Pigmented Fruits and Vegetables: Removes polar organic acids, some sugars and lipids, and carotinoides and chlorophyll; not for use with planar pesticides	100 – 2 mL tubes	50 mg PSA 50 mg GCB 150 mg MgSO₄Part No. 5982-5222, 5982-5222CH	25 mg PSA 2.5 mg GCB 150 mg MgSO₄Part No. 5982-5221, 5982-5221CH
	50 – 15 mL tubes	400 mg PSA 400 mg GCB 1200 mg MgSO₄Part No. 5982-5258	150 mg PSA 15 mg GCB 885 mg MgSO₄ Part No. 5982-5256, 5982-5256CH
Highly Pigmented Fruits and Vegetables: Removes polar organic acids, some sugars and lipids, plus high levels of carotinoides and chlorophyll; not for use with planar pesticides	100 – 2 mL tubes		25 mg PSA 7.5 mg GCB 150 mg MgSO₄Part No. 5982-5321, 5982-5321CH
	50 – 15 mL tubes		150 mg PSA 45 mg GCB 855 mg MgSO₄ Part No. 5982-5356, 5982-5356CH
Fruits and Vegetables with Pigments and Fats: Removes polar organic acids, some sugars and lipids, plus carotinoides and chlorophyll; not for use with planar pesticides	100 – 2 mL tubes	50 mg PSA 50 mg GCB 150 mg MgSO₄50 mg C18EC Part No. 5982-5421 5982-5421CH
	50 – 15 mL tubes	400 mg PSA 400 mg GCB 1200 mg MgSO₄400 mg C18EC Part No. 5982-5456 5982-5456CH
Other Food Methods: Removes biological matrix interferences, including hydrophobic substances (fats, lipids) and proteins	100 – 2 mL tubes	25 mg C18 150 mg MgSO₄ Part No. 5982-4921 5982-4921CH
	50 – 15 mL tubes	150 mg C18 900 mg MgSO₄Part No. 5982-4956 5982-4956CH
PSA = Primary Secondary Amine; GCB = Graphitized Carbon Black; C18EC = Octadecysilane, end-capped (For this step, the AOAC method specifies 1 mL or 6 mL aliquot samples; the EN method recommends 1 mL or 8 mL aliquot samples. Kits are assembled based on these specifications).

Dispersive SPE Kits
Kit	Quantity & Size/Pack	Contents & Part No. CH - with Ceramic Homogenizers
Universal kits for fruits and vegetables: Removes virtually all matrix interfering materials including: polar organic acids, lipids, sugars, proteins, carotinoids and chlorophyll.	100- 2ml	50 mg PSA 50 mg C18 7.5 mg GCB 150 mg MgSO4 Part no. 5982-0028 5982-0028CH
Universal kits for fruits and vegetables: Removes virtually all matrix interfering materials including: polar organic acids, lipids, sugars, proteins, carotinoids and chlorophyll.	50/bx	400mg PSA 400mgC18 45mgGCB 1200mg MgSO4 Part no. 5982-0029 5982-0029CH

Agilent now offers custom QuEChERS dispersive kits to meet your specific needs. Complete this form for a custom quote. QuEChERS Kit Brochure