Agilent | TOP Cartridges

For DNA, RNA and Trityl-on Oligonucleotide Purification

TOP, TOP-DNA and TOP-RNA cartridges provide a high throughput, simple, cost effective solution for DNA and RNA oligonucleotide purification. The TOP product range incorporates a unique 96-well plate with removable tubes, streamlined gravity flow or vacuum procedure, and proprietary polymeric resin. Agilent's innovative technology delivers superior yield and purity for standard oligos up to 1 μmol synthesis scale and up to 150-mer in length. Flexibility is assured from a choice of simple gravity flow (for walk-away and low initial set up cost) or vacuum procedure (for fast turn around - less than 15 minutes for the whole purification process). Up to 10 minutes drying time between each step is permissible with no effect on the purification results.

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Superior yield and purity come from the proprietary polymeric resins and optimized buffers
Typical yield is more than 85% and typical purity is over 90%, eliminating the need for multiple sample loading steps
Agilent TOP cartridges use up to two thirds less reagent than products from other vendors

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