LC and LC/MS Column Selection Flow Chart
To use the column selection guide diagram below, simply follow the path for your analyte and mobile phase. At the far right, click on your final column selection to view detail information.
SAMPLE
Molecular Weight < 2000
Organic Solvent Soluble
Hexane Soluble
Normal-Phase Mode with Bare Silica
Normal-Phase Mode with Bonded-Phase
MeOH and MeOH:H20 or ACN and ACN:H20 Soluble
Reversed-Phase Mode with Bonded-Phase
Gel Permeation (Small Molecule)
THF Soluble
Water Soluble
Non-Ionic
Ionic
Reversed-Phase Mode with Bonded-Phase Using Ionization Control
Reversed-Phase Mode with Bonded-Phase Using Ion-Pair Agent
Reversed-Phase Mode with Bare Silica
Ion-Exchange Mode
Molecular Weight > 2000
Gel Permeation Chromatography
Gel Filtration Chromatography
Ion-Exchange Mode with Wide-Pore Material
Reversed-Phase Mode with Wide-Pore Material