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Protein analysis holds the promise of a new era of pharmaceutical discovery. But it can be challenging, and difficult. Sometimes it feels like the technology hasn’t kept up with the needs of scientists.
Proven technology
The new columns are packed with ZORBAX StableBond technology with C18, C8 and C3 functionalities, and an end-capped diphenyl to give you:
• Stability at low pH – you can run your protein and peptide separations down to pH 1 using trifluoroacetic acid (TFA) and formic acid eluents with complete confidence.
• Temperature stability – you can run your separations up to 80 °C to improve efficiency and reduce eluent viscosity, without compromising column lifetime.
StableBond media is based on the ZORBAX silica, designed to minimize strong adsorption of basic peptides and proteins, and deliver symmetrical peaks with minimal peak tailing.
The diphenyl phase is a proven chemistry taken from the Pursuit 200Å columns and applied to the wider pore ZORBAX 300Å columns to enable the benefit of this unique selectivity to be used for the analysis of larger proteins.
C18, C8, C3 and diphenyl functionalities
• C18 – bonding a sterically-protected C18 functionality, diisobutyl n-octadecylsilane, to the ZORBAX particle produces a chemically stable stationary phase for long column lifetimes when using acidic mobile phases. This is important for the analysis of peptide fragments from protein digests and small proteins.
• C8 – the sterically protected C8 functionality, diisopropyl n-octylsilane, gives improved separation and recovery for larger proteins, including antibodies.
• C3 – broadens the selectivity, peak shape and recovery options for larger proteins, including antibodies.
• Diphenyl – gain the benefit of additional selectivity through pi-pi interactions with aromatic amino acids.
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Get your copy of the new BioHPLC Column Selection Guide
 
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Flyer:
- Increase your productivity with with Agilent ZORBAX RRHD 300Å 1.8 μm columns
Click here to download the flyer
Primers:
- Agilent's Guide to Recombinant Protein Characterization
- Compliance for Biopharmaceutical Laboratories
Click here to download the primers
Application Notes:
- Analysis of oxidized insulin chains using reversed-phase Agilent ZORBAX RRHD 300SB-C18 1.8 µm
- Analyze MAb and BSA digests by UHPLC with UV detection and Agilent ZORBAX RRHD 300SB-C18 1.8 µm
- Physicochemical characterization of a therapeutic protein by peptide mapping, SEC and IEX using the Agilent 1260 Infinity Bio-inert Quaternary LC system
- Comparison of hydrophilic polymer coated 3 μm and 5 μm 300Å silicas for the separation of MAb aggregates
- Reversed-phase separation of intact monoclonal antibodies using
Agilent ZORBAX Rapid Resolution High Definition 300SB-C8
- Fast separation of recombinant human erythropoietin using
Reversed-phased Agilent ZORBAX RRHD 300SB-C18, 1.8 μm
Click here to download any of these application notes
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With four different ligand types, C18, C8 and C3 alkyl chains, and the diphenyl to provide additional selectivity based on pi-pi aromatic amino acids. Agilent has the widest range of reversed-phase columns for all peptide and protein UHPLC separations. |
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