Over the years, numerous studies have been published by collaborators which demonstrate the very high correlation in results between RapidFire-MS and LC-MS.
The charts below are from these publications and feature RapidFire/MS and LC/MS comparisons for analyses of CYP inhibition, microsomal Stability, PAMPA and PPB assays.
Please contact us if you have a specific compound or reaction you would like to analyze using RapidFire technology.
CYP Inhibition: Correlation of RF-MS to LC-MS/MS
Data from 8 different enzyme/substrate pairs and 1 to 3 inhibitors for each pair conducted using traditional LC-MS at BD Biosciences and RapidFire technology at BIOCIUS. The dotted line represents a line of unity.
References:
Elke S. Perloff, Shangara S. Dehal, Andrew K. Mason, Andrew P. Blanchard, William A. LaMarr, Can C. Ozbal, Vaughn P. Miller, Charles L. Crespi, and David M. Stresser, Comparison of RapidFire® Ultra High Throughput LC/MS/MS with Traditional LC/MS/MS for Cytochrome P450 Inhibition Testing, Presented at ISSX 11th European Regional Meeting, 2009, Lisbon, Portugal.
Microsomal Stability: Correlation of RapidFire-TOF to LC-MS/MS
Correlation of microsomal stability results for 597 discovery compounds. Assays were performed at Novartis using Novartis compounds and analysis was compared by using LC-MS/MS and a RapidFire 360 interfaced to an Agilent 6530 Q-TOF. The R2 values show very high agreement between the data sets.
References
Hatsis P, Romm M, Miller VP, Amin J, LaMarr WA, Ozbal C, & Harriman S. (2010) Reducing Bottlenecks in ADME Sample Analysis using Solid Phase Extraction with a Quadrupole Time-of-Flight Mass Spectrometer. 58th ASMS Conference on Mass Spectrometry and Allied Topics, Salt Lake City UT.
Sample Microsomal Stability Assay Results Using RapidFire
PAMPA: Correlation of RapidFire-TOF to LC-MS/MS
PAMPA assay results using the BD Gentest Pre-coated PAMPA plates and analysis by a RapidFire 360 interfaced to an Agilent 6530 Q-TOF. A subset of the experimental data for which there were corresponding literature values (Chen et al. 2008) is shown. The R2 values show very high agreement between the data sets.
References
Romm MV, Parikh N, Miller VP, LaMarr WA, Ozbal CC (2010) Evaluation of Accurate Mass TOF-MS for Use in High Throughput PAMPA & Plasma Protein Binding Screens, 58th ASMS Conference on Mass Spectrometry and Allied Topics, Salt Lake City UT.
Chen X, Murawski A, Karishma P, Crespi CL, Balimane PV (2008) A Novel Design of Artificial Membrane for Improving the PAMPA Model. Pharmaceutical Research 25(7): 1511-1520.
PPB: Correlation of RF-TOF to LC-MS/MS
Plasma Protein Binding results using the Rapid Equilibrium Dialysis Device (RED), Thermo Scientific Pierce and analysis by a RapidFire 360 interfaced to an Agilent 6530 Q-TOF. Data shown is a small subset of the experimental data for which there were corresponding literature values (Waters et al. 2008). The R2 values show very high agreement between the data sets.
References
Romm MV, Parikh N, Miller VP, LaMarr WA, Ozbal CC (2010) Evaluation of Accurate Mass TOF-MS for Use in High Throughput PAMPA & Plasma Protein Binding Screens, 58th ASMS Conference on Mass Spectrometry and Allied Topics, Salt Lake City UT.
Waters, NJ, Jones R, Williams G, Sohal B (2008) Validation of a Rapid Equilibrium Dialysis Approach for the Measurement of Plasma Protein Binding, J Pharm Sci. 97(10): 4586-95.
Sample Plasma Protein Binding Results
