A Multiplexed Panel in <10 Seconds per Sample.
The RapidFire High-throughput MS System is widely used by laboratories in HTS and ADMET programs in order to maximize resources and shorten results turnaround time. However, RapidFire is also a promising tool for forensic toxicology and other clinical research programs to analyze blood, plasma, urine, food, and pesticides.
For example, a recent clinical research application note describes how RapidFire technology, in combination with time-of-flight mass spectrometry, simultaneously multiplexed an entire panel of benzodiazepines in <10 seconds. The study also found a very high correlation with LC-MS/MS methods, with the RapidFire (whether in multiplexed or single runs) delivering the same results 10x faster.
 Benzodiazepines
Detection of benzodiazepines is important but complex. Benzodiazepines are extensively metabolized, and the parent compounds are not easily found in urine samples. A typical approach to benzodiazepine detection involves a multi-step, labor intensive process. An immunoassay-based screening assay method is used followed by, when necessary, chromatography-based methods (i.e., GC/MS, HPLC, or LC/MS) for confirmation and quantitation. However, chromatographic separation is challenged by the simultaneous detection and quantification of multiple benzodiazepines due to their diverse biochemical properties including polarity.
With RapidFire, researchers were able to perform accurate, precise, simultaneous – and single run -- analyses of all 9 benzodiazepines in urine with data acquisition at 9.5 seconds.
For more information or to explore the possibilities of your research application, please contact us.
The information presented herein is intended for research use only and not intended for diagnostic purposes.
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