The ability to collect multiple levels of MS data in a single run is one of many advantages of an ion trap. The 6300 Series ion traps' sophisticated software contains a wide array of static and active data-dependent acquisition features that help you maximize the amount of useful data acquired from each run. For simplicity and convenience, all MS/MS data acquired from a run are stored in a single file.
| Data-Dependent Acquisition Features |
| Feature |
Benefit |
| N most abundant parents |
Increases the number of unique precursor ions from which data are acquired. Can be set separately for MS>2. Especially helpful for coeluting peaks. |
| Isotopic exclusion |
Prevents acquisition of unnecessary MS/MS data from isotopes (C13 etc.) |
| Static include list |
Ensures data is acquired from target ions, even if they are present at low abundance |
| Static exclude list |
Eliminates acquisition of MS/MS data from solvent ions and other background components |
| Static preferred list |
Ensures data is acquired from target ions, if present, but allows acquisition of data from other ions if target ions are not present |
| Preferred charge-state selection |
Ensures selection of doubly charged peptide ions for higher quality MS/MS data |
| Active exclusion – repeat count |
Increases the amount of unique data acquired by preventing acquisition of MS/MS data from the same ion more than a user-specified number of times |
| Active exclusion – exclude time |
Removes ions from the active exclusion list after a user-specified time to ensure reacquisition if an ion appears in more than one chromatographic peak |
| Threshold – absolute |
Ensures data is acquired only from ions of user-specified abundance |
| Threshold – relative |
Ensures data is acquired only from ions of user-specified abundance relative to the most abundant ion in an MS scan1 |
| Neutral loss detection |
Identifies neutral losses in MS/MS and triggers MS3 or pseudo-MS3 for additional structural information. Especially useful for analysis of phosphopeptides. |
| Stages of MS |
 |
Automated |
Manual |
| 6310 |
5 |
11 |
| 6320 |
5 |
11 |
| 6330 |
5 |
11 |
| 6340 |
5 |
11 |
Better MS/MS data through collision-energy rampingCollision-induced dissociation (CID) occurs between each stage of MS in MS n analyses. The 6300 Series ion traps' unique SmartFrag collision-energy ramping ensures that every precursor ion receives exactly the energy it needs for optimum fragmentation. The result is greater product ion generation and more structural information from fewer stages of MS.
Maximum resolution for unique, quality dataThe 6300 Series ion traps' impressive array of data-dependent acquisition options combines with its excellent scan speed and mass resolution to provide you with a unique performance option: maximum resolution scan mode. Using data-dependent acquisition criteria, the ion trap scans at maximum resolution over a narrow mass window around each selected ion. The result is the best of all worlds, very high quality data from more unique ions. You can use maximum resolution scan mode for both MS and MS/MS analyses.
Fast polarity switching for increased throughputThe ability to collect complementary positive- and negative-ionization data in a single run can save you precious time and sample. The fast (approximately 0.5 second) polarity switching time of the 6300 Series ion traps means they can collect more scans over a peak for better data quality and sensitivity.
Smart parameter settings provide expert results for non-expertsNot every user wants or needs the complication of setting every ion trap parameter. To simplify operation, the ion trap software includes a "smart" mode. Based on simple information such as target mass, compound stability, and expected distribution of ions relative to the target ion, the software automatically determines the optimum ion trap parameter settings. You still have full, independent control over ion source settings for complete compatibility with LC methods. |