Specifications - Agilent 1200 Series LC Micro Collection/Spotting System 

Agilent 1200 Series LC Micro Collection/Spotting System specifications

Performance specs

Baseline noise:
±3 × 10-5 AU, at 254 nm, at flow rates <100 µL/min

System delay volume (typical)

5 µL from EFC to column head, for flow rates up to 20 µL/min (default setup); 14 µL from EFC to column head, for flow rates up to 100 µl/min (default setup)

System control

Through local computer software, LAN or local handheld control module

GLP features

Early maintenance feedback—EMF
(tracks lamp burn time, usage, number of injections with limits and feedback messages)

Agilent 1200 Series Micro Collector/Spotter

Delay volume

Typically 0.25 µL with 25 µm capillary 1 µL with 50 µm capillary 4 µL with 100 µm capillary

384 and 96 well-plate format (standard and conical shape),
eppendorf tubes (0.5, 1.5, 2.0 ml)

MALDI carriers

For Agilent, Applied Biosystems, Bruker and Micromass targets

Fraction capacity

4 well plates, 4 MALDI plates or 4 x 27 Eppendorf tubes

Maximum collector capacity

2 micro fraction collector/spotter in parallel with 2PS/6PT micro valve 

Typical volumes for spotting

100 - 5000 nL

Spotting frequency

< 0.33 Hz

Matrix addition

With syringe pump

Maximum. system flow

100 µL/min (higher flow rates requires system modifications)

Minimum fraction volumes

Typically 2 µL (depending on fraction collection container)

Cooling

With G1330B Peltier Thermostat

Trigger modes

Time and peak-based (threshold, up-/downslope and time table), combination of different modes

Trigger source

Agilent 1200 Series MWW and DAD detectors and third-party detectors (requires UIB)

Environment

Specifications Agilent 1200 Series Modules