Read what customers have to say about the Agilent HPLC-Chip/MS system!
- "The Agilent Phosphochip is the ultimate ease-of-use tool for phosphopeptide analysis as it has a fully integrated microfluidic design that enables routine phosphopeptide analysis, without the hassles of clogging and plumbing in conventional nanoflow LC"
Professor Albert J.R. Heck, Utrecht University, Netherlands
- "For our biomarker discovery project at the Institute of Systems Biology, the ability to generate highly reproducible LC-MS and LC-MS/MS patterns of complex peptide samples is of critical importance. The reproducibility of LC-MS patterns achieved with our samples by Agilent scientists using the new HPLC-Chip system is substantially better than what we have been able to achieve with traditional capillary LC technology. The HPLC-Chip represents a significant advance in the separation tools available to proteomics researchers."
Professor Rudolf Aebersold, Institut für Molekulare Systembiologie, Switzerland
- "In addition to the ease of use, ruggedness and reliability of the HPLC-Chip/nanoflow LC interface, this microfluidic system offers a convenient modular platform for multidimensional separation, including affinity selection. The reproducibility and sensitivity of the HPLC-Chip/nanoflow LC coupled to either an ion trap or time-of-flight mass spectrometer provides a powerful protein identification and expression platform to any proteomics and drug discovery programs."
Professor Pierre Thibault, Université de Montréal, Canada
- "For long-time veterans of nanospray and proteomics, the Agilent Chip Cube is like Christmas morning. Forget the fused silica, the flame-pulled tips and pressure cell packing. Just slide in a spray chip and you’re good to go!"
David K. Crockett, ARUP Institute for Clinical and Experimental Pathology, USA
- "Using the HPLC-Chip, we have more than doubled our productivity, analyzing four to six samplers per hour instead of the one or two that we could do with classical nanoflow-LC, and all at increased sensitivity."
Dr. David Stapleton, Bio21 Molecular Science and Biotechnology Institute, Australia
- "It is easy to perform nanoflow-LC ES-MS analysis using the Agilent HPLC-Chip system. Not only does it simplify the process but also produces more reliable data."
Prof. Ming-Ren Fuh, Soochow University, Taiwan
- "The primary problem for LC/MS glycomics has been the difficulty in achieving stable and sensitive nanoscale electrospray over the long time periods required for analysis of large sample sets. The Agilent HPLC-Chip/MS system solves this problem by virtue of an engineered device that contains the trapping cartridge, nano-column, microfluidic connections, and electrospray emitter. Using this system, one can produce LC/MS data of the quality necessary to reach significant conclusions in biomedicine."
Prof. Joseph Zaia, Boston University Center for Biomedical Mass Spectrometry, USA
- "Scientists should spend their time in the lab doing meaningful biological experiments, not searching for leaks and dead volumes in their nanoflow-LC system. The Agilent HPLC-Chip is the solution, making nanoflow-LC for high sensitive protein and PTM identification easy, reliable, stable, and reproducible, far exceeding the traditional setup. Our results with the Agilent HPLC-Chip and 6520 QTOF MS verify this improvement in the daily work and free hands and brains for biological experiments."
Dr. Manfred Raida, Experimental Therapeutic Center, Singapore
- "I love the Agilent Chip Cube set up! Before we moved to the Chip Cube, nanospray was such a pain and it could only be managed by experienced users. Now, we can train someone to run their own samples in a few hours. One of the best things about the Chip Cube is the reliability. We can consistently go through an entire tray of samples and have the same sensitivity for the hundredth sample as we did for the first. When I started using nanospray, everyone had to pull their own needles and pack their own columns. I can't tell you how many times I would set up a series of samples only to have the needle clog or the current drop out on me. The Agilent HPLC-Chip system works every time. With low complexity samples such as in-gel digestions from 2D gels, we can do a complete LC run in 12 minutes. Being able to do 5 samples an hour, with improved sensitivity, has changed the way we pick spots – we just do them all."
Prof. Brian Bothner, Montana State University, USA
- "The Agilent Chip Cube is wonderful for our facility, since we train graduate students, postdocs and technicians to run their own samples and to understand the analysis process in more detail than would be possible in a service mode. The Chip Cube provides improved chromatographic resolution and sensitivity due to the low dead volumes in the fluidics. The Chip Cube removes problems of cross contamination between users since each project has its own separation chip."
Prof. Edward Dratz, Montana State University, USA
- “The reproducibility and reliability of the Agilent HPLC Chip system combined with the power of repetitive exclusion runs on the 6500 Q-Tof has allowed me to dig deeper into proteomes than I ever thought was possible.”
Dr. Nicolas L Taylor, University of Western Australia, Australia
- "The introduction of the HPLC chip technology represents a quantum leap in the development of nano-LC. The improved detection sensitivity and reproducibility are obvious advantages. However, the key benefit to our laboratory has been the significant increase in productivity since people with no experience in nano liquid phase separations readily can perform nano-ESI-MS analysis on a routine basis using the Agilent chip system."
Dr. Tasso Miliotis, AstraZeneca, Sweden
 HPLC-Chip/MS System
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