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Specifications Agilent 1200 Series Fluorescence Detector |
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Performance |
Single wavelength operation:
10 fg anthracene LOD, Ex 250 nm, Em 400 nm
H20 >500 Raman, Ex 350 nm, Em 397 nm,
dark value 450 nm, standard flow cell
Dual wavelength operation:
H20 > 300 Raman, Ex 350 nm, Em 397 nm and Ex 350, em 450 nm, standard flow cell |
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Light source |
20 W Xenon flash lamp, normal mode: 20 W
economy mode: 5 W |
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Pulse frequency |
365 Hz for signal mode, 73 Hz for economy mode |
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Excitation monochromator |
Concave holographic grating, F/1.6, blaze 300 nm, range 200-700 nm and zero-order, bandwidth 20 nm |
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Emission monochromator |
Concave holographic grating, F/1.6, blaze 400 nm, range 280-900 nm and zero-order, bandwidth 20 nm |
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Photomultiplier |
200-600 nm, exchangeable |
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Reference system |
Inline excitation measurement |
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Time programing |
Up to four signals, response time, PMT gain, baseline behavior (append, free, zero), spectral parameters |
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Spectral acquisition |
Excitation or emission spectra, scan speed 28 ms per datapoint, e.g. 0.6 s per spectrum 200-400 nm, 10 nm step |
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Step size |
1-20 nm |
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Spectra storage |
All |
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Wavelength repeatability |
±0.2 nm |
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Wavelength accuracy |
±3 nm |
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Flow cells |
Standard: 8 µL volume, 20 bar (2 MPa) pressure maximum, quartz.
Optional: fluorescence cuvette for offline spectroscopic measurements with 1 mL syringe, 8 µL volume, quartz |
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Control and data evaluation |
Agilent ChemStation for HPLC or Agilent 1100 Series control module without spectral data analysis |
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Environment |
0 - 40 °C constant temperature at <95% humidity (non-condensing) |
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GLP |
Early maintenance feedback (EMF) for continuous tracking of instrument usage, display of feedback messages in terms of lamp burn time with user-settable limits and feedback messages. Electronic records of maintenance and errors. Verification of wavelength accuracy using Raman band (H20) lines. |
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