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Protein 80 Kit |
Protein 230 Kit |
High Sensitivity
Protein 250 Kit |
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Analytical specifications |
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Sizing range |
5-80 kDa |
14-230 kDa |
10-250 kDa |
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Typical sizing resolution |
10% |
10% |
10% |
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Typical sizing accuracy |
10% CV (CAII, BLG) |
10% CV (BSA, CAII) |
10% CV (BSA) |
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Sizing reproducibility |
3% CV (CAII, BLG) |
3% CV (BSA, CAII) |
3% CV (BSA) |
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Sensitivity
(Signal/Noise > 3) |
6 ng/μL CAII (15 ng/μL BSA) in PBS, 10 ng/μL (CAII) in 0.5 M NaCl
(30 ng/μL BSA in 0.5 M NaCl) |
6 ng/μL CAII (15 ng/μL BSA) in PBS
30 ng/μL (BSA) in 0.5 M NaCl |
1 pg/μL (labeled BSA)
in water on chip
5 pg/μL (labeled BSA)
in PBS on chip
Labeling reaction at
1 ng/µL of total protein |
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Quantitative range |
60-2000 ng/μL CAII in PBS |
15-2000 ng/μL CAII, 30-2000 ng/μL BSA in PBS |
up to 4 orders of magnitude (0.3 to 3000 ng/µL BSA) |
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Qualitative range |
6-4000 ng/μL CAII and BLG |
6-5000 ng/μL CAII, 15-5000 ng/μL BSA in PBS |
- |
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Quantitation reproducibility |
20% CV (CAII, BLG) |
20% CV (BSA, CAII) |
20% CV (BSA) |
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Protein 80 Kit |
Protein 230 Kit |
High Sensitivity
Protein 250 Kit |
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Physical specifications |
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Analysis run time |
30 minutes |
25 minutes |
30 minutes |
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Number of samples |
10 samples/chip |
10 samples/chip |
10 samples/chip |
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Sample volume |
4 μL |
4 μL |
5 μL |
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Kit stability |
4 months (for storage temperature see individual box) |
4 months (for storage temperature see individual box) |
6 months at -20° C |
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Compatible buffers |
List of compatible buffers |
List of compatible buffers |
List of compatible buffers |
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CAII = Carbonic Anhydrase, BSA = Bovine Serum Albumin, BLG = beta-Lactoglobulin |
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