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2100 Bioanalyzer - DNA Solutions
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2100 Bioanalyzer - DNA Solutions
Sizing, quantification and quality control of DNA
The Agilent 2100 Bioanalyzer DNA 1000, DNA 7500 and DNA 12000 kits allow you to automatically size and quantitate PCR fragments and restriction digests accurately and reproducibly. PCR and RT-PCR are among the most widely used techniques in molecular biology. In some cases, it is sufficient to detect the presence or absence of a PCR product, while in many cases quantification of this product and detection of non-specific amplification is critical. The Agilent 2100 Bioanalyzer is ideal for automated sizing and quantification of PCR and RT-PCR products in single or multiplex mode with unprecedented accuracy.
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Features
Sizing accuracy
– normalization to two internal markers and ladders
Quantitation accuracy and reproducibility
– automated quantitation of each DNA fragment against internal standards
High resolution of a large number of bands
– critical for any multiplex PCR application
Broad linear dynamic range
– enables the detection of less abundant products, e.g. low abundance messages in multiplex RT-PCR amplifications or non-specific amplifications
Sensitivity
– Laser Induced Fluorescence (LIF) detection for fragments down to 0.1 ng
Minimal sample consumption
– only 1 μL of material required per analysis
Ready-to-use
– standardized assays and pre-packaged reagent kits
Application Areas & Software
Amplicon quality in forensic DNA studies
Gene expression quantification by RT-PCR
DNA QC for comparative genomic hybridization
Detection of genetically modified organisms GMOs
DNA sizing and quantification applications
2100 Expert software
Additional Information
High Sensitivity DNA Kit – highest sensitivity for sizing, quantification and quality control of complex DNA samples
Agilent 2100 Bioanalyzer - for sizing, quantification and quality control of DNA, RNA, proteins and cells
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New Applications
Performance characteristics of the High Sensitivity DNA Kit
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