Specifications 7100 Capillary Electrophoresis System
| Pressure system |
| Programmable with -100 to +100 mbar on inlet |
| Flushing with 1 bar or with high pressure 2–12 bar |
| Vial pressurization with high pressure 2–12 bar on inlet and/or outlet |
| Safety features |
| Current leak detection low current limit |
| Liquid leak sensor |
| Safety sensors at door and cover disabling high voltage |
| Vial sensor |
| Interfaces |
| LAN, CAN, RS232, remote control, analog in (1V, 20 bit, integrated A/D converter), analog out |
| Injection modes |
| Self correcting injection system with injection from inlet or outlet |
| Programmable range |
up to 10,000 seconds |
| Pressure |
–100 to +100 mbar |
| Electrokinetic |
–30 to +30 kV |
| Autosampler/ fraction collector |
| 50-position carousel |
| All vials are randomly accessible from inlet or outlet end of capillary |
Temperature control with external waterbath with vial temperature from 10–40 °C
(non-condensing conditions, minimum waterbath temperature +1 °C) |
| Replenishment |
| Satellite station for refilling of inlet or outlet buffer vials with fresh buffer for automatic, continuous operation. |
| Selectable buffer levelling. |
| Vials |
| 100 μL (polypropylene or glas) with resealing snap caps |
| 1 mL (polypropylene) with resealing snap caps |
| 2 mL (glas) with resealing snap caps |
| Capillary cassette |
| High-speed forced-air cooler with Peltier element |
| Temperature range |
10 °C below ambient, up to 60 °C (min. 10 °C cassette temperature) |
| Minimum total capillary length |
33 cm |
| Capillary compatibility 365 µm o.d. |
| Detector |
| Real time UV-Visible diode-array detector (190–600 nm) |
| Temperature controlled |
| Wavelength accuracy |
1 nm |
| Response time |
0.025 to 10 s |
| Light source |
prealigned deuterium or high brightness lamp |
| Signals |
up to eight signals simultaneously, full spectral acquisition with Agilent ChemStation |
|
Sensitivity |
1 μM 4-hydroxy-aceto-phenon injected at 50 mbar*5 sec, 3 x 50 µm bubble cell capillary, signal/noise >6* (50 mM borate buffer pH 9.3, 25 kV) |
| Baseline noise |
<50 μAU (at 2 sec response time)* |
| Linear dynamic range |
1x104 (3x50 μm bubble cell capillary)* |
| Raw data channels |
| Detector signals and spectrum, voltage, current, leak current, power, cassette temperature, pressure, lamp voltage and detector temperature |
| Diagnostic functions |
| RFID tag for lamp, early maintenance feedback, supported by Agilent Lab Advisor software with integrated diagnosis test suite |
| System control |
| Operating with graphical user interface under Windows XP (SP3) |
| Time programmable parameters |
Time programmable parameters: voltage, current, power, polarity, pressure, inlet and outlet vial, capillary temperature, pre and post-run conditioning with pressure and/or voltage, Replenishment, fraction collection** |
|
Electrophoresis power |
|
Voltage range |
setable 0 to ± 30 kV supply |
|
Current |
setable 0–300 μA |
|
Power |
setable 0–6 W |
|
Operation under constant voltage, current or power |
|
Programmable polarity switch |
| CE specific software |
| Mobility report, time corrected areas, pI calibration and bio polymer size calibration |
| Dimensions |
| Width |
35 cm |
| Height |
59 cm |
| Depth |
51 cm |
| Weight |
35 kg |
| Environment |
| Temperature |
5–40 °C |
| Humidity |
up to 80 % relative humidity at 31 °C (non-condensing) |
| Power requirements |
| Line voltage |
100-240 V, max. 300W |
| Line frequency |
50-60 Hz |
* typical value
**upgradeable
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