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Quick Amp Labeling Kit, two-color 

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Reagents 
Amplify and label target RNA 
The Agilent Quick Amp Labeling Kit, two-color contains reagents to amplify and label target RNA to generate complementary RNA (cRNA) for oligo microarrays used in gene expression profiling and other downstream analyses. Resarch use only.

Also available:  Low Input Quick Amp Labeling Kit, two-color.
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Related Information

Features
  • Sufficient reagents for 20 reactions
  • Sample required per reaction is 50 ng – 5 µg total RNA or 10 – 200 ng poly A(+) RNA
  • Labeling reaction produces 0.75 – 2.0 µg of cyanine-labeled cRNA (anti-sense) from as little as 50 ng of total RNA, depending on RNA source
  • Sensitive detection limit of 1 mRNA copy in 6.9M transcript when used in the Agilent microarray workflow
  • Process time is approximately 6 hours, instead of overnight
    (for cRNA generation) as with other commercially available kits
  • Contents include 11 reagents and Cyanine 3-CTP and Cyanine 5-CTP dyes.
  • Kit should be stored at –20°C

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