Processing of miRNA microarray requires the following Agilent reagents and hardware.
Reagents
|
Processing Step |
Product Name |
Use |
|
Sample Labeling & Hybridization |
miRNA Complete Labeling and Hyb Kit |
Contains a novel Cyanine 3-nucleotide along with all necessary array labeling and hybridization reagents. Contains sufficient reagents to label and hybridize 24 microarrays. Kit contents include T4 RNA Ligase, Calf Intestinal Phosphatase, Cyanine 3-pCp, Hi-RPM Hybridization Buffer, DMSO and associated buffers. |
|
Microarray Wash |
Gene Expression Wash Buffer Kit |
Contains two 5188-5325 (Gene Expression Wash Buffer 1, 4L each), one 5188-5326 (Gene Expression Wash Buffer 2, 4L) and Triton X-102 detergent additive. Kit contains sufficient wash buffer for up to 80 slides. Wash buffers may be purchased separately |
Hardware
|
Hardware Type |
Product Name |
Description |
|
Sample QC |
2100 Bioanalyzer System (optional) |
Includes 2100 Bioanalyzer with HP/Compaq desktop PC, printer, 2100 expert software, vortex mixer, accessories, without cartridge and license. |
|
RNA 6000 Nano LabChip Kit (optional) |
Includes reagents, supplies, 25 LabChips, 2 cleaning chips, syringe, and reagent kit guide. |
|
Hybridization Chambers & Accessories |
Hybridization Chamber - SureHyb |
Includes chamber base, chamber top, clamp, chamber thumbscrew, plastic tweezers and a microarray hybridization user guide. |
|
Hybridization Backing Kit |
Includes gasket slides for the eight-plex microarrays in kit sizes of 5, 20, or 100 gaskets. |
|
Hybridization Oven & Accessories |
Hybridization Oven |
Equipped with a removal rotator rack, and designed with user-settable rotator speeds and variable hybridization temperatures. |
|
Hybridization Chamber Rotator Rack |
Rotator rack for holding hybridization chambers during incubation in hybridization oven. |
|
DNA Microarray Scanner |
Scanner bundle |
Includes: DNA microarray scanner, 48-position carousel with cover, 2 slide holder kits (50 slide holders), crossover LAN cable, Feature Extraction and Analysis software, PC data system, installation and familiarization, and one-year service warranty and technical support. |
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