Processing of the DNA Methylation microarrays can require additional reagents and hardware not included in the kit. Tables below list those items.
Reagents
|
Processing Step |
Product Name |
Use |
|
Sample Labeling |
Genomic DNA Enyzmatic Labeling Kit |
Contains Cyanine 3- and Cyanine 5-dUTP and reagents to label genomic DNA for Oligo aCGH microarrays. Kit is sufficient for 50 reactions (or100 reactions for the 8-pack). Customer must purchase the following to purify the labeled DNA: Microcon YM-30 filter units (Millipore part number 42410) or GE Healthcare plates (part number 25-9005-98). |
|
Microarray Hybridization |
Oligo aCGH/ChIP-on-chip Hybridization Kit |
Contains 2X Hi-RPM Hybridization Solution and 10X Blocking Agent for 25 slides. Customer must purchase the following to prepare hybridization solution: Human Cot-1 DNA (Invitrogen part numbers 15279-011 or 15279-101 and Kreatech part number EA-020) or Mouse Cot-1 DNA (Invitrogen part number 18440-016) or Rat Hybloc (Applied Genetics part number RHB). DNase/RNase-Free Distilled Water (Invitrogen part number 10977-015). |
|
Oligo aCGH/ChIP-on-chip Hyb Kit, Large Volume |
Contains 2X Hi-RPM Hybridization Solution and 10X CGH Blocking Agent for 100 slides. Customer must purchase the following to prepare hybridization solution: Human Cot-1 DNA (Invitrogen part numbers 15279-011 or 15279-101 and Kreatech part number EA-020) or Mouse Cot-1 DNA (Invitrogen part number 18440-016) or Rat Hybloc (Applied Genetics part number RHB). DNase/RNase-Free Distilled Water (Invitrogen part number 10977-015). |
|
Microarray Wash |
Oligo aCGH/ChIP-on-chip Wash Buffer Kit |
This kit contains Oligo aCGH Wash Buffer 1 (4L, part number 5188-5221) and Oligo Wash Buffer 2 (4L, part number 5188-5222).
This kit is available in two configurations:
Option 1 for manual processing contains 2 containers of Wash Buffer 1 and 1 container of Wash Buffer 2. The kit is sufficient for processing up to 40 oligo aCGH slides.
Option 2 for Tecan processing contains 1 container of Wash Buffer 1 and 2 containers of Wash Buffer 2. The kit is sufficient for processing up to 120 oligo aCGH slides. |
|
Stabilization and Drying Solution
|
Contains reagent that prevents ozone-induced degradation of cyanine dyes during processing and scanning of Agilent oligo-based microarrays.
Sufficient reagent for up to 25 oligo aCGH slides. Customer must purchase the following to use this product with oligo aCGH arrays: Acetonitrile (Sigma-Aldrich 271004-1L). |
Hardware
|
Hardware Type |
Product Name |
Description |
|
Sample QC |
2100 Bioanalyzer System
(optional) |
Includes 2100 Bioanalyzer with HP/Compaq desktop PC, printer, 2100 expert software, vortex mixer, accessories, without cartridge and license. |
|
RNA 6000 Nano LabChip Kit (optional) |
Includes reagents, supplies, 25 LabChips, 2 cleaning chips, 1 syringe, and reagent kit guide. |
|
Hybridization Chambers & Accessories |
Hybridization Chamber - SureHyb
|
Includes chamber base, chamber top, clamp, chamber thumbscrew, plastic tweezers and a microarray hybridization user guide. |
|
Hybridization Backing Kit
|
Includes gasket slides for the x 44K microarrays in kit sizes of 5, 20, or 100 gaskets. |
|
Hybridization Oven & Accessories |
Hybridization Oven
|
Equipped with a removal rotator rack, and designed with user-settable rotator speeds and variable hybridization temperatures. |
|
Hybridization Chamber Rotator Rack
|
Rotator Rack for holding hybridization chambers during incubation in hybridization oven. |
|
DNA Microarray Scanner |
Scanner bundle
|
Includes: DNA microarray scanner, 48-position carousel with cover, 2 slide holder kits (50 slide holders), crossover LAN cable, Feature Extraction and Analysis software, PC data system, installation and familiarization, and one-year service warranty and technical support. |
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