Figure 4.
Conditions
|
|
Column
|
ZORBAX RRHD SB-C18, 2.1 mm, 1.8 µm
|
|
Mobile phase
|
A = 0.1% formic acid
B = acetonitrile with 0.1% formic acid
|
|
Gradient
|
10-100% B in 30 min
|
|
Flow rate
|
0.4 mL/min
|
|
Detector
|
UV, 280 nm
|
|
Temperature
|
Ambient
|
|
Sample
|
Licorice root extract
|
|
Abbreviations
|
Rs = resolution
nc = peak capacity
|
A longer 150 mm ZORBAX RRHD column enabled a high-resolution separation of this complex sample of licorice root.