Figure 3.
Conditions
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Final method - Agilent 1290 Infinity LC System
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Column
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BEH C18, 100 mm x 2.1 mm, 1.7 µm
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Mobile phase
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A = 0.25% w/w ammonium acetate in water
B = acetonitrile
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Flow rate
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0.66 mL/min
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Gradient
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0-0.1 min: 5% B isocratic
0.1-3.45 min: 5-57.5% B
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Temperature
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32°C
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Injection volume
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1 µL
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Detection
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DAD, signal 275/4 nm, reference 400/60 nm
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Maximum pressure
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880 bar
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The analysis on the 1290 Infinity LC took only 3.2 minutes, which was four times faster than the original method.
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