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Microarray Data Analysis - Level I
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450
Euro
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R1795A |
1 Tag/e |
Praktische Übungen |
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690
Sfr. zzgl. gesetzl. MWSt. |
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Beschreibung: |
Level I focuses on the basics of microarray data analysis and workflow. Topics include: data import, normalization, quality control methods, filtering, gene list manipulation, complex experiment specification, fold change analysis, finding differentially expressed genes, and data export. You will learn why normalization is important for microarray data and when to apply certain normalizations, such as global scaling and intensity-dependent Lowess, to your own one- or two-color data sets. You will also learn about integrating gene expression data with known biological annotations available in public and proprietary databases. Finally, you will learn how to export interesting microarray results that can be used for presentation. |
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Kursüberblick: |
Morning
GeneSpring terminology and interface review
Installing Genomes and Importing Data
Annotation Tools
• Updating genome annotations
• Gene Ontology (GO) Browser
Normalizations
• 1-color experiments
• RMA and GC-RMA pre-processing of Affymetrix data
• 2-color experiments
Afternoon
Sample-level Quality Control
• Box Plots
• Find Similar Sample tool
• Scatter Plot
Gene-level Quality Control
• Filter on Flags
• Filter on Expression Level
• Filter on Data File
Gene Lists and Venn Diagrams
Gene List Manipulation
• Edit gene lists
• Filter on gene list numbers
Importing and Exporting Data in GeneSpring
• Exporting gene lists
• Exporting graphics for publication
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Voraussetzungen: |
Read the Tutorial in GeneSpring (Help menu >Tutorial) to become familiar with the interface. Begin exploring features using sample data that comes with GeneSpring. Proficiency using computers and navigating in common software applications is assumed. |
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Geräte: |
Agilent provided laptop computers loaded with the current version of GeneSpring software. |
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Zielgruppe: |
Scientists and technicians that seek to gain knowledge in the basics of microarray data analysis and workflow using GeneSpring software. |
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Kalender - Kundenschulung. Um sich für einen Kurs anzumelden, klicken Sie auf den Termin/Ort. |
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On Demand
Weitere Informationen
Dieser Kurs findet nur in englischer Sprache statt.
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