| Description: |
The objective of Level II training is to teach users the different methods of identifying mass features of biological interest. This includes
applying statistical analysis methods to identify mass features that are differentially expressed between experimental groups, performing clustering analysis to group mass
features according to the similarity of their abundance profiles, and identifying mass features of interest using Metlin and Spectrum Mill database search. Users will
also learn other features in GeneSpring MS that will allow users to interrogate each mass feature of interest in greater details. This includes the Mass Inspector
window and the Mass Detail view. |
| Outline: |
- Morning
- Statistical Analysis
- • One-way Tests
- • Two-way Tests
- • Multiple Testing Correction
- • Post-hoc Tests
- • Volcano Plots
- Fold-change Analysis
- Afternoon
- Similarity Metrics
- • Find Similar Mass Tool
- Clustering Analysis
- • K-means Clustering
- • Self-organizing Map (SOM) Clustering
- • Quality Threshold (QT) Clustering
- Metabolite and Protein Identification
- • Metlin Metabolite Database Search
- • Spectrum Mill Protein Mass Fingerprinting Database Search
- Exporting Inclusion List for QTOF MS/MS analysis
|
| Prerequisites: |
GeneSpring MS - Level I (R1710A). |
| Equipment: |
Agilent provided laptop computers loaded with the GeneSpring MS software. |
| Student Profile: |
Scientists and technicians that seek to gain knowledge in using GeneSpring MS software to identify mass features of biological interest. |
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