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Course Schedule |
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Advanced Agilent 6410 QQQ LC/MS/MS Course
| $4,000(USD) |
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R1657A |
Four Days |
Hands-On Operation |
| $4,000(CAD) |
| Description: |
This lecture and laboratory course covers advanced concepts in API electrospray and APCI LC triple quadrupole LC/MS/MS method development,
method optimization, instrument operation, qualitative determinations and quantitative analysis. The majority of the class is devoted to hands-on instrument
operation, supplemented with laboratory experiments using student samples. The course is customized towards the students needs with emphasis on methods development
to improve sensitivity, minimizing sample suppression and interferences, optimization, specificity and improving sample throughput. The student will learn
tricks and tips that can aid them in future method development and optimization. |
Note
Students are encouraged to bring samples to this training for method development exercises.
| Outline: |
- Initial Method Development
- - Choosing mode of ionization
- - Polarity for detection
- - Fragmentor Optimization
- - Collision energy optimization
- HPLC and Sample Prep Considerations
- Review of initial results
- Sensitivity Improvement
- - Customize tune conditions
- - Customize instrument settings
- Specificity Improvement
- - Ions to monitor
- - Chromatographic issues
- - Overcoming matrix interference
- Quantitation
- - Achieving precision
- - Achieving accuracy
- - Linearity
- - LOQ
- Improve sample throughput
- Method Robustness and performance
- - Is it ready to be validated?
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| Prerequisites: |
The Agilent (R1893A) training course is required. |
| Equipment: |
An Agilent 6410 QQQ system with Mass Hunter. |
| Student Profile: |
The course is designed for the operator who wants to understand the advanced concepts and operation of Electrospray and APCI 1200 Rapid
resolution LC 6410 Triple quadrupole MS/MS. The course is tailored for operators who need to know how to better utilize the QQQ system in
the development of methods, optimization of methods, and for qualitative and quantitative determinations. |
| Course Calendar: | Durham, NC | October 27, 2008 - October 30, 2008 |
| Durham, NC | December 08, 2008 - December 11, 2008 |
| Durham, NC | February 23, 2009 - February 26, 2009 |
| Durham, NC | September 14, 2009 - September 17, 2009 |
| Durham, NC | December 07, 2009 - December 10, 2009 |
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