Mass Spectrometry



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Agilent 1100 LC/MSD Trap Advanced Applications


$3,238(USD) ATP H8982A Four Days Hands-On Operation
$3,773(CAD)
Description: This lecture and laboratory course covers advanced concepts in Electrospray and APCI LC/ Ion Trap MSn method development, method optimization, instrument operation, qualitative determinations and quantitative analysis. The majority of the class is devoted to hands on instrument operation with laboratory experiments on the Agilent 1100 Series LC/MSD Trap (applicable to the 6300 Series Ion Traps as well) that emphasize the topics covered in the lectures. The course is customized towards the students needs in the areas pharmaceuticals, clinical, biomolecule, industrial and environmental applications and it is encouraged that students bring their own samples to work on in the class.
Outline:
Advanced ion formation processes in Electrospray and APCI
Understanding the Ion Trap and MSn
Advanced Electrospray and APCI LC/Ion Trap MSn operation
Optimizing the LC/MSD TRAP method for your analysis
Developing a method for qualitative determinations
Interpretation of the qualitative results-Interpretation of MSn CID mass spectra
Developing a quantitation MS/MS method using external standards
Developing a quantitation MS/MS method using isotopically labeled internal standards
Increasing sample throughput
Developing and optimization of capillary LC/ ion trap MSn
System maintenance and diagnostics
Solving problems in proteomics
Prerequisites: The Agilent (H8966A) training course or 6 months operation of the Agilent 1100 or 6300 Series Ion Trap system is required.
Equipment: An Agilent 1100 LC/MSD Trap system with ChemStation.
Student Profile: The course is designed for the operator who wants to understand the advanced concepts of Electrospray and APCI LC/ ion trap MS and ion trap MSn and how to better utilize the Agilent 1100 and/or 6300 Series LC/MSD Ion Trap system in the development of methods, optimization of methods and for qualitative and quantitative determinations.

Course Calendar:Durham, NC  September 30, 2008 - October 03, 2008
Durham, NC  December 15, 2008 - December 18, 2008