Liquid Chromatography



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Introduction to Liquid Chromatography and the HPLC ChemStation


$1,971(USD) H5929A Four Days Hands-On Operation
$2,395(CAD)
Description: This four day laboratory and lecture course is designed to introduce users to the practical aspects of performing routine analyses using the Agilent 1100/1200 Series Liquid Chromatographs with the HPLC ChemStation. Topics include instruction in the basics of liquid chromatography, instrument maintenance, calibration tables, sequencing, and chromatographic troubleshooting.
Outline:
Introduction to High Performance Liquid Chromatography
Practical HPLC
HPLC Instrumentation
Lab: LC Hardware and the Diagnosis View
Common Troubleshooting
Introduction to the HPLC ChemStation and Acquisition
Lab: Acquisition Methods
Chromatographic Figures of Merit
Qualitative Data Analysis
Lab: Qualitative Data Analysis
Quantification - Concepts and Integration
Lab: Integration
Quantification - Calibration
Lab: Quantification
Basic Reporting
Sequence and Batch Review
Lab: Sequence and Batch Review
Selecting an HPLC Column
HPLC Operating Parameters
Lab: Comparison of High Speed, Small Bore, and Analytical Columns
Lab: Optimize the Separation
Prerequisites: None
Equipment: Agilent 1100/1200 Series Liquid Chromatographs with HPLC (3D) ChemStation
Student Profile: Anyone responsible for routine operation and maintenance of the Agilent 1100/1200 Series with HPLC ChemStation. The basics of liquid chromatography will be provided as well as software operation. If you are responsible for method development see H1186A and H4033A.

Course Calendar:Alpharetta, GA  November 11, 2008 - November 14, 2008

Follow-on Courses:
  • H8969A - Agilent 1100/1200 Series HPLC Troubleshooting and Maintenance