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Course Schedule |
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Techniques of High Performance Liquid Chromatography
| $2,220(USD) |
H1186A |
Four Days |
Hands-On Operation |
| $2,622(CAD) |
| Description: |
This hands-on laboratory and lecture course is designed to increase the participants understanding and ability to apply the modern practices of High Performance Liquid Chromatography. The participant will learn how to select appropriate instrumentation and HPLC columns, optimize separations, and interpret and troubleshoot chromatograms. Participants will learn how theoretical considerations will help increase their ability to produce robust methods and solve analytical problems. HPLC practices and evaluation of system performance will help students increase their efficiency and data quality. The hands-on aspect of the course will allow a novice HPLC user to gain important practical operational experience with the HPLC.
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| Outline: |
- Day One
- Introduction to HPLC
HPLC Instrumentation
- Day Two
- Practical HPLC Requirements
- The Separation
- Gradient Elution
- Day Three
- HPLC Troubleshooting
- Quantitative and Qualitative Analysis
- Column Hardware
- Reversed-Phase HPLC
- Day Four
- Ion-Pair HPLC
- Ion Exchange
- Normal Phase
- Size Exclusion
- Method Development
- Method Validation
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| Prerequisites: |
None; however, a working knowledge of PC's is recommended. |
| Equipment: |
The Agilent 1100/1200 Series Liquid Chomatographs with HPLC ChemStation
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| Student Profile: |
A neophyte user of Agilent liquid chromatographs who has responsibility for routine sample
analysis and may have responsibility for developing new methods. |
| Course Calendar: | Alpharetta, GA | September 09, 2008 - September 12, 2008 |
| Alpharetta, GA | December 02, 2008 - December 05, 2008 |
Follow-on Courses:
- H4039A - Agilent HPLC (3D) ChemStation Data Analysis and Reporting
- H5928A - Agilent HPLC (2D) ChemStation Data Analysis and Reporting
- H4033A - Agilent HPLC (3D) ChemStation Operation
- H8969A - Agilent 1100/1200 HPLC Troubleshooting and Maintenance
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