42nd Congress of European Societies of Toxicology
Crakow, Poland
Agilent Technologies Satellite Symposium Novel Approaches to Toxicological Research
Tuesday, September 13, 2005
13:00 - 14:30
Join Agilent Technologies and CIT for an informative workshop on cutting edge applications using novel tools for toxicological research.
Agilent Technologies is leading the way to faster and more automated, high throughput approaches to analyzing bio-molecules using chip-based technologies, such as microarrays, microfluidics, and the world's first HPLC-chip. Agilent and CIT jointly present the seminar 'Novel Approaches to Toxicological Research', featuring the latest technologies and approaches for toxicological research. Practical research and technological advances will be discussed.
ATTN: Register today - places are limited! Featured Speakers and Topics
Specific procedures and robust RNA quality controls: the key factors for a successful toxicogenomics approach.
Toxicogenomics approaches reveal toxicity-specific biomarkers that can allow the elucidation of toxicologic mechanisms and help the risk assessment processes.
The technologies involved in such approaches can be performed at a low or a high throughput scale by the use of quantitative RT-PCR or cDNA microarrays, respectively. However, one of the key point for the success of these approaches is the quality of the starting material, i.e. total or messenger RNAs.
RNAs are very labile molecules that can be very rapidly degraded after the animal sacrifice, especially in RNAses-rich tissues like pancreas. Thus, we have developed specific necropsy procedures for the collection of more than 50 rat, 20 mouse and 20 primate tissues while keeping RNA integrity. RNA quality and concentration is routinely determined by analyzing samples aliquots loaded on RNA LabChip® with the Agilent 2100 bioanalyzer. This lab-on-a-chip system requires only small amounts of total RNAs, allowing the analysis of material extracted from very small tissues like mouse nerves or arteries. Moreover, RNA quality is assessed by automatically calculating the 28S and 18S ribosomal RNA peak ratios.
The use of specific necropsy and RNA extraction procedures as well as relevant quality controls allows us to have very reproducible results on DNA chips experiments with an inter-animal variability less than 2% on the whole transcriptome. Agilent 2100 bioanalyzer. This lab-on-a-chip system requires only small amounts of total RNAs, allowing the analysis of material extracted from very small tissues like mouse nerves or arteries. Moreover, RNA quality is assessed by automatically calculating the 28S and 18S ribosomal RNA peak ratios.
The use of specific necropsy and RNA extraction procedures as well as relevant quality controls allows us to have very reproducible results on DNA chips experiments with an inter-animal variability less than 2% on the whole transcriptome
Head of Functional Genomics Department, CIT, Paris, FRANCE
Investigating the insulin-dependent contribution of xenobiotic metabolism and oxidative stress to hepatocellular carcinoma using microarray technology.
Worldwide, hepatocellular carcinoma (HCC) is the fifth most prevalent malignancy. It is estimated that HCC will cause nearly a half million deaths annually. Alarmingly, the incidence of HCC is rapidly rising, particularly in developed countries. Over the past thirty years the number of HCC cases has nearly doubled in the United States. It has recently been established that type II diabetes is associated with a three-fold increase in risk for HCC. With the hypothesis that diabetes may contribute to HCC carcinogenesis through insulin-dependent regulation of phase I and II drug-metabolizing enzymes, as well as genes involved in modulating oxidative stress, we used Agilent microarrays to characterize the effect of insulin and ketone body levels (acetoacetate) on global gene expression, oxidative stress, and signaling pathways in primary cultured rat hepatocytes. Additionally, analysis of microarray data using samples obtained from polysomal fractionation provides evidence of translational control of several important genes, including cytochrome P450s. This research has been supported by National Institute of Environmental Health Sciences grants ES03656 and ES06639.
Institute of Environmental Health Sciences, Wayne State University, Detroit, Michigan, USA
Systems Genomics - A Foundation For The Study of Toxicogenomics
Toxicogenomics combines the analysis of genome-wide mRNA expression profiling, cell and tissue-specific protein expression, and metabolite profiling with conventional toxicology in an effort to understand the role of environmental agents in gene-based disease. In a similar fashion, a portfolio of microarray applications for the complete understanding of genomic events enables Systems Genomics. This presentation provides an overview of the Agilent complete solution for the analysis of DNA and RNA target classes, including offerings for array-based comparative genomic hybridization (aCGH), location analysis, splice variant analysis, microRNA profiling, and DNA methylation. Each of these applications will be discussed in the context of toxicogenomics research.
Agilent Technologies Inc, Palo Alto, California, USA
Program
13:00 - 13:05
Welcome & Introduction
Dr. Wim Dorlijn, Agilent Technologies
13:05 - 13:30
Speaker 1 - Specific procedures and robust RNA quality controls: the key factors for a successful toxicogenomics approach. Dr. Philippe Ancian, CIT, Paris, France
13:30 - 13:55
Speaker 2 - Investigating the insulin-dependent contribution of xenobiotic metabolism and oxidative stress to hepatocellular carcinoma using microarray technology Professor Alan A. Dombkowski
Institute of Environmental Health Sciences, Wayne State University, Detroit, Michigan, USA
13:55 - 14:20
Speaker 3 - Systems Genomics - A Foundation For The Study of Toxicogenomics Dr. Steven R. Kain,
Agilent Technologies Inc, Palo Alto, California, USA
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