| Septum Material | Compatible with | Incompatible with | Resealability | Max. Temp. |
| Rubber (Natural/Butyl) | ACN, acetone, DMF, alcohols, diethylamine, DMSO, phenols | Chlorinated solvents, aromatics, hydrocarbons, carbon disulfide | Excellent | < 100°C |
| PTFE/Natural or Butyl Rubber | PTFE resistance until punctured, then septa or liner will have compatibility of rubber | Good | < 100°C | |
| Silicone/Silicone Rubber | Alcohol, acetone, ether, DMF, DMSO | ACN, THF, benzene chloroform, pyridine, toluene, hexane, heptane | Excellent | < 200°C |
| PTFE/Silicone, PTFE/Silicone/PTFE | PTFE resistance until punctured, then septa will have compatibility of silicone | Average | < 200°C | |
| VITON | Chlorinated solvents, benzene, toluene, alcohols, hexane, heptane | DMF, DMSO, ACN, THF, pyridine, dioxane, methanol, acetone | Good | < 260°C |